Second-harmonic imaging microscopy of living cells
نویسندگان
چکیده
منابع مشابه
Second-harmonic imaging microscopy of living cells.
Second harmonic generation (SHG) has been developed in our laboratories as a high-resolution nonlinear optical imaging microscopy for cellular membranes and intact tissues. SHG shares many of the advantageous features for microscopy of another more established nonlinear optical technique: two-photon excited fluorescence (TPEF). Both are capable of optical sectioning to produce three-dimensional...
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PURPOSE The purpose of this study was to evaluate the ability of second-harmonic imaging to identify differences in corneal stromal collagen organization between normal human and keratoconus corneas. METHODS Six normal corneas from eye bank donors and 13 corneas of patients with keratoconus, obtained after penetrating keratoplasty were examined. A femtosecond titanium-sapphire laser with 800-...
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ژورنال
عنوان ژورنال: Journal of Biomedical Optics
سال: 2001
ISSN: 1083-3668
DOI: 10.1117/1.1383294